The research revealed the dental status of patients with verified helminthiasis. Toxocarosis was associated with pathologic changes in the oral mucous of the patients. Dental care of patients with erosive-ulcerative form of lichen planus in the course of antiparasitic therapy lead to reduction of the affected area in two times

The RT-PCR samples of blood plasma and leucocytes taken from 20 in-door patients in 2015 in the city of Khabarovsk with suspected tick-borne infections contained Borrelia miyamotoi DNA and Rickettsia species DNA up to 10% and 30% respectively. The subsequent sequencing of the genome sites identified the affiliation of Rickettsia DNA to the pathogenic to people Rickettsia heilongjiangensis and specificity of Borrelia DNA to B.miyamotoi. The fact of PCR- based diagnostics of tick-borne recurrent fever caused B.miyamotoi and Far Eastern tick fever caused by R.heilongjiangensis was shown among the patients with early stage of the disease. The screening of the Lyme’s ticks collected within Khabarovsk Krai area shows the circulation of R. heilongjiangensis and B. miyamotoi in the part of the Russian Far East.

Twenty-three patients with median age of 37,4±4,05 suffering from hemorrhagic fever with renal syndrome (HFRS) were studied. All patients were examined for the levels of selenium in the blood serum and free radical oxidation (FRO) indices with the biochemicoluminescence method. The tests revealed that during the period of early apyrexia in patients with HFRS was registered significant decrease of selenium level in blood serum as compared to the period of late convalescence and control group. At the same time, there were no significant difference between selenium concentration during the period of late convalescence and in the control group. The research revealed significant moderate inverse correlation between the level of selenium in blood serum and FRO indices. This finding reflects intensity of the process of free radical oxidation in the blood serum.

Among diagnostic techniques, methods of molecular-genetic analysis are currently applied more and more often because they allow to study genome of the infectious agent. Due to a high genetic variation of entero-, rota-, noroviruses, human immunodeficiency virus, hepatitis B and C viruses during the process of their evolution different genetic reconfigurations occur that lead to generation of new intratypic genovariants. It is now possible to establish interrelations in the foci of abovementioned infections, evaluate of ubiquity or multiplicity of infection sources. Should be noted the importance of molecular-genetic techniques in investigation of hospital-acquired diseases particularly during investigation of hospital acquired human immunodeficiency virus (HIV-1) and hepatitis B and C viruses. Monitoring of genotype circulation of the abovementioned viruses allows to discover new epidemic variants of infectious agents in the population, detect importation of international strains in Russia, which can be also registered in Khabarovsk region.

Etiological reading and interpretation of pneumonias is one the most critical challenges of the diagnostics due to usage of various substrata and research methods, complexity of interpretation of the findings, related to risk of contamination of the clinical material (sputum) with the oropharyngeal microflora, as well as upper airways during sampling process; and insufficient sensitivity of classical bacteriological research methods and other factors [1]. Etiological reading and intepretation of community-acquired pneumonia was conducted at the virological laboratory utilizing PCR focused at wide-range of causative agents. For the purposes of research, nasal, oral cavity and throat smears were taken from the patients in the healthcare facilities. The material was provided for the year 2015 and the first four months of the year 2016. It is demonstrated that the prevailing pathogen, during all periods, was Streptococcus pneumoniae (pneumococcus), it was identified among 65.9-82.3% of screened patients. The following viral agents were prevalent during nonepedemic period: rhinoviruses and RS-viruses; during the epidemic surge of the influenza and ARVI, the influenza viruses were the most active. Comparative valuation of the diagnostics resultы was conducted, it was found that pneumococcus as well as RS-virus are more frequently identified among junior age groups patients.

The research presents materials on epidemiologic investigation of food toxicoinfection outbreak of Staphylococcal etiology in Sakha (Yakutia) Republic. Due to disregard of personal hygiene rules, sanitary-epidemiological and disinfection regime as well as cooking technology of confectionery products occurred a bacterial contamination of baked confectionery products because of bacterial carriers of pathogenic Staphylococcus (Staphylococcus aureus) that were working in the pastry shop. Patisseries contained metabolic by-products of Staphylococcus aureus - staphylococcal toxin, which was the reason of food poisoning. In order to determine the source of the infection was performed molecular-genetic identification of Staphylococcus aureus. Withdrawal of production of “Nariyaana” pastry shop allowed to neutralize the breakout at shortest time and stop the spread of infection among population.

The article presents results of detection of the hepatitis virus E (HVE) infection markers (anti-HVE IgM, Ig G, RNA HVE) among 1547 labor migrants that arrived in Russian Federation recently. Among them citizens from Uzbekistan totaled 464 people, from Tadzhikistan - 415, Ukraine - 522, Moldova- 146. Among them 5,93% (79/1333) were seropositive for anti-HVE IgM, 25,36% (338/1333) - for HVE IgG, 3% (40/1333) hag both anti HVE IgM and IgG, RNA HBV was not detected. Anti-HVE Ig G was detected more frequently among migrants from Uzbekistan and Tadzhikistan (25,4% and 43,1%) as compared to the migrants from Ukraine and Moldova (7,8% and 12,3%). Consequently, considerable amount of labor migrants that arrive in Russian Federation have anamnestic antibody response to HVE. The range of these indexes reflect levels of endemicity of particular country the migrants are from. High frequency of anti-HVE IgM detection indicates on present or recent infection. This allows to imply that there is a high risk of HVE importation on the territory of the Russian Federation. The received data indicates on necessity of including screening for acute HVE infection in to the algorithm of routine examination among migrants.

Research objective: investigate correlation between the levels of HBsAg, hepatitis B virus (HBV) DNA and ɑ-fetoprotein in patients with diagnosis of chronic HBeAg-negative viral hepatitis B. Materials and methods: samples from 89 patients with diagnosis of chronic viral hepatitis B were studied during the first half of 2016. Laboratory assessment was performed in FBIS “Khabarovsk research institute of epidemiology and microbiology” of Rospotrebnadzor. The samples were diagnosed for the presence of HBV DNA, serological markers of hepatitis B (qualitative and quantitative evaluation of HBsAg, antibodies to HBcAg, Ig M antibodies to HBcAg, HBeAg and antibodies to HBeAg); antibodies to hepatitis D and quantitative evaluation of ɑ-fetoprotein Results and discussion: after conducting correlation analysis between levels of HBsAg and HBV DNA in blood serum of patients with diagnosis of chronic HBeAg-negative viral hepatitis B were obtained mixed results. The research revealed negative correlation between HBsAg and ɑ-fetoprotein levels. Conclusion: quantitative evaluation of HBsAg in blood serum of patients with diagnosis of chronic HBeAg-negative viral hepatitis B is not informative for estimation of level of HBV DNA. Further study is needed in order to confirm received data on negative correlation between HBsAg and ɑ-fetoprotein levels in order to investigate their interference.

The current review presents background information on influence of viral hepatitis B, C and D on the course of HIV-infection according to the latest worldwide and nationwide molecular epidemiological studies.

HIV infection continues to be one of the global issues of practical healthcare. Currently it has claimed lives of more than 34 million people. Currently the outspread of HIV infection in Russia has grown into an epidemic. In the Far Eastern federal district (FEFD) the total number of HIV positive people has reached 20 990 people. Accession rate of HIV infected people averages to 10,2±0,26% annually. Percentage of heterosexual transmission of HIV infection is also rising. The percentage of HIV positive people shifts to group of people of 30-40 years and older. Preventive registration indices total up to 89% of the HIV infected people.

Youth is disposed to high-risk sexual behavior. This is why prophylaxis issues of sexual (heterosexual) transmission route of HIV-infection should be taken in consideration during planning of the prophylactic measures. Behavioral patterns of youth regarding HIV-infection could be markers for development and timely correction of public-awareness campaigns in order to reduce the spread of HIV. In 2016 was conducted a latest social research among teenagers and youth (14-19 years old) of Khabarovsk region during which were analyzed 502 resultative questionnaires in comparison to the data of similar inquiries of prior years. During the analysis of the questionnaires was recovered an increasing present of the youth that are afraid of HIV-infected people and believe in myths considering the transmission routs of HIV-infection. This fact arises a certain amount of apprehension considering the discussed issue. In order to organize a systematic and high-quality prophylactic work among younger generation it is necessary to promote cooperation between governmental bodies concerning youth policy and education

Manifestation results of influenza and other acute respiratory viral infections epidemic process in the influenza epidemic season of 2015-2016 in Yekaterinburg are presented. The quantitative indexes of surveillance over influenza demonstrated that А(H1N1v) influenza virus prevailed in the epidemic process over the assessedperiod; the incrementwas the highest in 0-to-2 and 3-to-6 age groups and equaled 544.6% and 517.4% respectively.

Antiviral activity of substances and drugs (organosiliconzinc glycerohydrogel, In-58 peptide, human placenta hydrolysate, polyoxidonium) administered to laboratory animals using various methods against А/PR/8/34 (H1N1) influenza virus was studied. The studies showed that organosiliconzinc glycerohydrogelhad the most pronounced antiviral effect as distinct from the other drugs under test included into the experiment on laboratory mice.

The article presents the processes of free radical oxidation in the blood serum of young children with acute intestinal infections of viral and bacterial etiology. It shows a decrease in the activity of antioxidant protection and anti-radical peroxide resistance of the substrate with different etiology of the disease. In addition, there is a significant increase in hydroxyl radical production in bacterial diarrhea.

The findings of the long-term research of the resident gut microbiota, microflora and prevalence of opportunistic pathogenic bacteria, as well as yeasts - Candida among the adult population of the city of Khabarovsk, a large industrial center of the Russian Far East, were stated. It is found that considerable prevalence rate of disbiotic disorders among adult population and expressed seasonal dynamics with increase in spring and autumn. The changes were shown in the nature of gut microflora imbalance. The major types of opportunistic pathogenic bacteria were found among adults on a regular basis. K. pneumoniae was most abundant in the structure of microbiocenosis, it weight was 23.6%. The considerable growth of Candida was displayed mostly in the summer time period. The existence of much deeper changes in microbiota during discharge of multicomponent microbial and/or microbial-fungal associations was identified. Was determined a high level (46.2%) of phagoresistant opportunistic pathogenic bacteria and their primary isolation from associations.