No 25 (2014)
ЭПИЗООТОЛОГИЯ
9-11 104
Abstract
Natural plague foci in the North Caucasus have been exposed to various emergencies. Humanitarian crises are accompanied by active people migration, inadequate hygiene-and-sanitary living conditions. Natural disasters reduce epizootic activity due to downsizing of carrier and vector numbers.
11-15 107
Abstract
Long-term transformation of flea multispecies communities of the Mongolian pika, the basic carrier of the plague agent in Mountain-Altai natural focus, is studied. It is shown that in three mesofoci there are processes of gradual change of the flea number and correlation of separate species. Communities of the Mongolian pika fleas in each mesofocus are characterized by the expressed specificity by quantitative characteristics of mass species.
15-18 136
Abstract
The current areal of Mongolian pika (Ochotona pallasii), the basic carrier of the plague agent in Mountain-Altai natural plague focus, was described in Southeast Altai. It was shown that the area of this animal distribution increased up to 1,5 times from 1978 to 2013. So, in 1978 the registered area of the small animal habitation was about 1430 km2 and it increased to 2200 km2 in 2013.
D. B. Verzhutski,
A. Ya. Nikitin,
N. I. Kovaleva,
N. F. Galatsevich,
N. A. Chumakova,
S. V. Tkachenko,
A. V. Chumakov
18-22 150
Abstract
There are no analogues in the world to disinfestation activities carried out in the Sagil valley (Tyva natural plague focus) in 1981-1985 meaning novelty approaches, the level of implementation and the obtained results. Activity extinction of the autonomous plague mezofocus has been observed almost 30 years. This is a clear indication of direct dependence of the plague enzootic process from flea number - the main infection vectors and keepers in the focus. Prolonged low number of these insects at the territory of the independent host population caused the complete elimination of the pathogen unable to exist in any other habitat.
Z. . Adjaasuren,
D. . Tserennorov,
Z. . Myagmar,
T. . Ganhuyag,
D. . Otgonbayar,
T. . Bayar,
D. B. Verzhutsky,
D. . Ganbold,
S. V. Balakhonov
22-25 203
Abstract
The comparative analysis of the natural plague foci activities in Mongolia and human plague sickness rate for the last decade (2004-2013) is described. It is shown that intensity in the general decrease of the plague foci activity and its human morbidity reduction in the country is geographically uneven. The possible reasons of such changes are considered.
26-27 103
Abstract
Data on epizootological and epidemiological manifestations of natural focal infectious diseases transmitted by Ixodes ticks before and after high water periods in the territory of the Amur region is analyzed.
28-30 148
Abstract
New scientific data indicating the wide spread of new Rickettsia species pathogenic for humans and environmentally connected with Ixodes ticks are obtained in Russia and Kazakhstan. Total spreading over 15 genotypes of tick-borne alpha1-proteobacteria is found. Rickettsiae scenery is necessary to study in the regions of Kazakhstan and the Crimean Federal District.
30-32 89
Abstract
Analysis of epizootological-epidemiological situation concerning natural focal infections in Southern and North Caucasian Federal districts of the Russian Federation in 2013 is presented.
33-35 153
Abstract
Compositional comparison of the ticks’(Acari: Ixodidae) fauna associations evolved in the territories of Russky Island and Ussuriysk National Park (Primorsky Krai) over the last quarter of the XX and at the beginning of the XXI centuries was based on our own observations and literature sources. It was proved that island associations of ticks are more susceptible to transformations.
36-38 136
Abstract
Results of inspection for tick-borne encephalitis virus in 11 Soums (districts) of three Mongolian provinces and the epidemiological analysis of human sickness rate are generalized
M. A. Tarasov,
N. P. Shestopalov,
A. M. Porshakov,
S. I. Tolokonnikova,
V. A. Yanovich,
P. V. Kopylov,
A. D. Vorontsova,
N. V. Popov,
V. P. Toporkov,
A. V. Toporkov,
I. G. Karnaukhov,
V. V. Kutyrev
39-42 128
Abstract
The data of epizootiological inspection in the Jewish Autonomous Region (JAR) territory exposed to the catastrophic flooding in August-September, 2013 are summarized. In the context of the disaster, the numbers of small mammals - carriers of dangerous zoonotic infections (hemorrhagic fever with renal syndrome, tularemia, leptospirosis, etc.) were evaluated; the efficacy of deratization activities was verified; a number of new ecological-epizootiological factors characterizing distribution of the rodents in the natural and anthropogenic biotopes was suggested.
ESTIMATION OF EFFICIENCY OF DISINFESTATION ACTIONS IN THE AMUR REGION AFTER THE FLOOD PERIOD IN 2013
L. P. Okunev,
A. A. Perepelitsa,
O. I. Korotkoruchko,
T. Yu. Nekhryuk,
E. N. Burdinskaya,
O. K. Lyalina,
A. V. Samchuk,
I. A. Boiko,
K. V. Krakhmalyov,
O. S. Kizima,
A. Ya. Nikitin,
S. A. Borisov,
I. M. Morozov
42-47 111
Abstract
Results of epizootological-epidemiological inspection validate the implementation of disinfestation actions in the Amur region. Its organization and extent are described. Efficiency of the executed preventive works is analyzed and estimated.
M. A. Tarasov,
N. P. Shestopalov,
A. M. Porshakov,
A. I. Udovikov,
M. M. Shilov,
V. A. Yanovich,
P. V. Kopylov,
A. D. Vorontsova,
N. V. Popov
47-50 133
Abstract
To perform comprehensive assessment of the aftermaths consequent to the large-scale flooding in the Amur-river region it was suggested seldom-used for epizootiological and epidemiological analysis factors such as: necrophagy and numbers of inter- and intra-population contacts of carriers habitant in the areas with the high risk of exposure of the population to dangerous zoonotic infections that positively correlate with the factors predetermining epizootic activity of the foci.
50-53 110
Abstract
Circulation of pathogenic Leptospira in small mammals was revealed at the inspected territories in the Amur region, Khabarovsk Krai and Jewish autonomous region during a high water in 2013. Dominating species of Leptospira carriers was a field mouse, prevailing Leptospira serogroup - Icterohaemorrhagiae.
53-55 324
Abstract
The reasons of vector-borne tularemia outbreak occurrences in the last decade (the Central Federal district, the Arkhangelsk region, Khanty-Mansiysk city) were analyzed. Epizootic activity of natural tularemia foci of inundated-swamp type in these territories and immunization state against tularemia at present were estimated. Recommendations for the epizootic and epidemic situation control in the territory of the natural tularemia foci were given to prevent epidemic (group disease) outbreaks.
56-58 103
Abstract
The epizootic situation in connection with tularemia occurrence in Khanty-Mansiysk Autonomous region (Yugra) in 2013 is analyzed.
58-60 178
Abstract
Small mammals (650 individuals) caught in the north-eastern districts of the Voronezh region were investigated for the presence of the tularemia antigen and Francisella tularensis DNA. The basic species of small mammals involved in the pathogen circulation in natural tularemia foci were revealed. The peculiarities of biotopic distribution of the infected mammals are discussed.
60-62 222
Abstract
An epidemiological analysis of the recorded tularemia cases in the Arkhangelsk region was conducted. Some features of natural tularemia foci manifestations at the present time were revealed. For the first time clinical-epidemiological analysis and laboratory findings revealed patients infected with two natural focal infections (tularemia, leptospirosis) that indicated the presence of combined natural foci of these infections in the Arkhangelsk region. Since the mass immunization against tularemia in the USSR (from the middle of the last century to the present days) the most effective preventive measure against tularemia is human vaccination with a live tularemia vaccine.
63-65 287
Abstract
The total area of the natural tularemia foci in Kazakhstan is 520 thousand km2 and its considerable part is located in frontier territory with Russia. Epizootic conditions of the Kazakhstan tularemia foci in frontier territories with Russia are analyzed from 2000 to 2014.
66-68 132
Abstract
The conditions promoting circulation of transmissible natural focal infections pathogens, were studied, including particularly dangerous in Caucasian Mineral Waters region of Stavropol Territory. Was defined range of pathogens these infections. Natural reservoirs of these diseases pathogens were established.
G. M. Dmitrieva,
N. Yu. Ochekovskaya,
N. D. Oreshkina,
N. G. Zvereva,
G. A. Evtushok,
T. G. Chepizhko
68-70 114
Abstract
Results of zoological-entomological monitoring of the natural tularemia foci and estimation of complex anti-epidemic actions implemented in cooperation with the experts from Irkutsk Antiplague Research Institute at examination of a tularemia case registered in area of Boguchansk hydroelectric power station in 2012 г are characterized the epidemic tularemia process in Krasnoyarsk Territory.
E. V. Putintseva,
V. P. Smelyansky,
V. V. Manankov,
V. A. Pak,
N. V. Boroday,
N. I. Pogasiy,
G. A. Tkachenko,
D. V. Viktorov,
V. A. Antonov
71-73 123
Abstract
One hundred ninety one human cases of West Nile fever infections were registered in 16 Russian regions in 2013 that was considerably lower than a year before. The same tendency of the intensity decrease in the epidemiological process was observed also at the North American continent. The West Nile fever outbreak in Serbia caused the rise of morbidity in the European countries. In 2013 West Nile fever virus of genotype 2 (lineage 2) was found to circulate in Volgograd and Saratov regions and genotype 1 (lineage 1) - in the Astrakhan region.
T. Yu. Krasovskaya,
E. V. Kazorina,
E. V. Naidenova,
E. A. Bilko,
Zh. A. Kasyan,
I. V. Terekhova,
A. M. Senichkina,
V. E. Kuklev,
S. A. Shcherbakova
73-75 122
Abstract
First patients with West Nile virus fever were registered in Saratov region in 2012.
76-77 92
Abstract
The information about epizootic rabies situation in the Buryat Republic, the measures conducted in the rabies foci and the actions directed to the prevention of rabies disease among humans and animals is presented.
77-79 108
Abstract
Rabies is registered in more than 150 countries and territories. Rabies mortality is more than 55,000 people per a year in the world. Nearly 1 million of animals die from rabies. Every year, worldwide more than 15 million people receive a post-exposure vaccination to prevent the disease. Yearly more than 10,000 people are bitten by animals and vaccinated after exposed rabies risk. In the last 10 years 653 humans were exposed to rabies risk infection, and 3 persons died in Mongolia. This is directly related to animal rabies cases determined by mapping and time period in our study.
N. V. Boroday,
V. A. Pak,
E. V. Putintseva,
V. P. Smelyansky,
V. V. Manankov,
N. I. Pogasiy,
K. V. Zhukov
79-81 111
Abstract
Monitoring of the population number and sampling of vectors is required to determine West Nile virus infection in the natural foci and to predict the development of the epidemiological situation. Results of application of the automatic traps (Mosquito Magnet Independence) for mosquito collection in natural West Nile virus foci in the Volgograd region are represented.
O. L. Bogomazova,
I. V. Bezgodov,
V. B. Uspenskyi,
N. A. Miryashkin,
M. M. Verhosina,
V. B. Kazanova,
T. N. Osipova,
E. G. Romanenko,
I. V. Kozlova,
G. A. Danchinova
82-84 122
Abstract
In the light of the information about quantity of tick bites and tick’s infectiousness by different infectious agents (2006-2013) in Bodaibo district of Irkutsk region were identified some areas with increased ticks activity. In popular recreation areas were found ticks infected with spring-summer tick-borne encephalitis, tick-borne borreliosis, monocytic ehrlichiosis, human granulocytic anaplasmosis. In connection with presence of natural tick-borne infections foci in Bodaibo district preventive measures have been suggested.
T. V. Mzhel’Skaya,
A. V. Kirillova,
A. G. Kovalskii,
N. P. Visochina,
O. E. Trotsenko,
E. N. Prisyazhnyuk,
I. G. Pivovarova
85-87 116
Abstract
Epidemiological data regarding weather conditions in 2013-2014 and observations for quantity of Ixodes ticks in hot spots in the South of Khabarovsk region are presented in the current article. The infection rate of ticks removed from the citizens of Khabarovsk City during preflood months in 2013 was compared to that in 2014.
МИКРОБИОЛОГИЯ И ЛАБОРАТОРНАЯ ДИАГНОСТИКА
A. N. Boldyrev,
S. A. Bodnev,
S. N. Sokolov,
T. P. Mikryukova,
V. V. Solodky,
V. A. Ternovoi,
Yu. V. Tumanov,
O. V. Pyankov,
A. P. Agafonov
88-90 128
Abstract
A kit comprising the key components solely of Russian manufacturers and vendors for PCR and detection of Middle Eastern respiratory syndrome coronavirus 1b gene was developed on the basis of the primers and a probe suggested by German Center for the Study of Infections (Deutsches Zentrum für Infektionsforschung, DZIF, Germany). The detection of a virus-specific amplicon was performed by measuring the fluorescence as the endpoint and the ultraviolet light after the gel electrophoresis in the presence of ethidium bromide. The reaction was conducted in an accelerated program in 15 (10) μl volume. This approach does not require expensive equipment and Taq DNA polymerase such as Platinum-Taq DNA polymerase.
S. V. Bakhmeteva,
N. M. Pukhovskaya,
N. I. Zdanovskaya,
L. I. Ivanov,
N. B. Belozerova,
O. M. Utkina,
Ya. A. Zhuravlev,
V. F. Larichev
91-93 206
Abstract
Decoding data of imported arbovirus fever cases in the Far Eastern region of Russia in 2011-2014 are represented. Denge fever diagnosis was confirmed in 56 patients; one patient with West Nile fever and one patient with Chikungunia fever were revealed. If the clinical diagnosis was confirmed, IFA was used to reveal IgM/IgG antibodies and RT-PCR. RT-PCR and the phylogenetic analysis of nucleotide sequences showed the presence of Denge type 1, 2 and 3 viruses. Five Denge type 1 and 3 virus strains were isolated using Vero E6 cell culture and newborn white mice.
T. I. Borisova,
E. A. Sidorova,
A. V. Sevostyanova,
M. V. Lemeshevskaya,
I. V. Kotova,
K. A. Belyh,
E. I. Andaev
94-96 112
Abstract
Laboratory examination of clinical material from 14 patients arrived from South Eastern Asia and received treatment in Irkutsk Regional Infectious Clinical Hospital with suspicion to Denge fever was performed. RNA of Denge virus type 3 was revealed in 3 patients by RT-PCR method. Denge virus type 1 was isolated from blood of a patient. M-class antibodies were detected in 3 patients, G-class antibodies - in 9 patients, and four-fold increase of antibody titers in binate sera was noted in 2 patients. Antibodies to Denge virus failed to find out in 2 patients. Diagnosis was confirmed in 12 patients by laboratory serological (IFА) and molecular-genetic methods (RT-PCR).
96-98 153
Abstract
Application of a diagnostic preparation «Immunochromatographic test system for Yersinia pestis» (State Research Center of Applied Microbiology and Biotechnology, Obolensk) was experimentally analyzed for serological plague diagnostics in suspensions of laboratory animal internals. The technique of a sample preparation was developed. This method is simple for implementation and easily reproduced; it is a quick test for detection of Y. pestis capsular antigen. The test-system can be applied both in laboratory and field conditions including the activities of a specialized anti-epidemic team on the basis of mobile complexes and an epidemic brigade in natural plague foci.
98-100 124
Abstract
EPIDEMIC VALUE OF SPRING WATERS IN THE WEST KAZAKHSTAN AREA Detection of the spring number in the West Kazakhstan area was continued and the degree of its catchment was defined. Spring water was examined for Vibrio cholerae presence. Insignificant role of the underground waters in the epidemiology of intestinal infections was concluded.
101-102 135
Abstract
The mass spectrum of cholera toxin is defined by MALDI-TOF MS (Matrix Assisted Laser Desorption/Ionization Time of Flight Mass-Spectrometry), specific peaks of mass spectra are revealed, the possibility of its definition in the environments (tap water) is studied.
BIOLOGICAL INDICATORS OF CHOLERA AND PARAHEMOLYTIC VIBRIO BACTERIOPHAGES USED IN ITS DIFFERENTIATION
103-106 148
Abstract
The basic features of bacteriophage differentiation in cholera and parahemolytic vibrios are defined. It raises a possibility for detection of species belonging of the phages. These signs can be used in the research purposes and for solving of the applied problems to improve the laboratory diagnostics. The general identification scheme is created for the bacteriophage differentiation in cholera and parahemolytic vibrios.
106-108 124
Abstract
Comparative morphometric analysis of the functional systems with the definition of a number of parameters characterizing the adaptive-compensatory processes in biomodels was additionally performed to improve the objectivity and information value of preclinical evaluation of anti-plague and -tularemia vaccines.
E. A. Solovyev,
L. V. Sayapina,
A. A. Goryaev,
N. A. Osina,
M. P. Rudnik,
D. S. Davydov,
V. P. Bondarev
109-111 140
Abstract
Variability of cultural, morphological, biochemical, genetic and immunobiological properties of Francisella tularensis 15 NIIEG strains dried in 1953, 1966, 1969, 1987, 1990 , 2003 and 2012 and stored in a National collection of pathogenic bacteria (Russia) was detected. These data confirmed the need for a continuing study to maintain the stability of the F. tularensis 15 NIIEG strain.
111-113 106
Abstract
The project of refillable electronic database of mass spectrometric protein profiles of brucellosis strains in the environment of the program MALDI Biotyper v 3.0 (Bruker Daltonics, USA) has been developed. On the basis of analysis of representative mass spectra of 39 Brucella strains, 12 possible genus-specific fragments in the mass range 2000 - 20000 Da were identified.
113-115 117
Abstract
Volgograd Plague Control Research Institute, Volgograd In this paper we studied protein production by Bacillus anthracis isogenic variants with different set of virulence plasmids. It was shown that homotypic strains differed by S-layer protein and protective antigen production that should be taken into account in the selection of producer strains.
E. A. Sidorova,
M. O. Gorina,
S. A. Borisov,
A. Ya. Nikitin,
A. V. Samchuk,
E. V. Kravets,
E. A. Razenkova,
T. Yu. Nekhruk,
O. P. Kurganova,
E. I. Andaev
116-118 126
Abstract
Human blood sera were collected and a number of small mammals was recorded in epizootological-epidemiological examination of the Amur region during a high water of 2013. It was established that a field mouse (Apodemus agrarius) prevailed in natural biotops and human settlements (68 %). Analysis of clinical and field samples revealed antibodies to the causative agent of haemorragic fever with renal syndrome in human blood sera (5,3 %) and RNA of Hantavirus complex in 7,2 % of small mammals.
E. Yu. Sapega,
O. E. Trotsenko,
O. P. Kurganova,
V. A. Ott,
N. V. Korshunova,
T. V. Korita,
V. O. Kotova,
V. A. Yanovich,
Yu. A. Garbuz,
P. V. Kopilov,
L. V. Butakova,
T. A. Zaitseva,
A. A. Perepelitsa,
T. N. Karavyanskaya,
E. N. Prisyazhnyuk,
E. M. Golubeva,
V. I. Reznik,
E. S. Mironenko,
L. A. Balahontseva,
A. P. Bondarenko,
A. N. Lukashev,
S. V. Balakhonov1O,
A. K. Noskov1O,
S. A. Kosilko1O,
N. A. Novikova,
L. N. Golitsina,
M. E. Ignat’Eva,
A. A. Rubtsova,
B. B. Darizhapov,
G. G. Onishchenko
119-127 143
Abstract
Flood emergency on the territory of Khabarovsk region, Amur Region and Jewish Autonomous Region necessitated wider application of molecular-genetic methods of typing and evaluation of origin of enteroviruses. The most commonly detected enteroviruses in 2013, such as Coxsackie B-5, A-6, A-9, A-16 and ECHO-6 were subjected to phylogenetic analysis. The level of genetic similarity and possibility of epidemiological relationship were analyzed for Enterovirus 71. Performed studies indicate different origin of enteroviruses including importation of viruses, most likely from countries of Asia-Pacific Region.
E. V. Prokhvatilova,
V. A. Antonov,
D. V. Viktorov,
V. I. Ilyukhin,
N. P. Khrapova,
G. A. Tkachenko,
I. B. Zakharova,
N. G. Plehanova,
I. V. Novitskaya,
M. Ya. Kulakov,
T. V. Zamarina,
I. I. Korsakova,
S. S. Savchenko,
O. S. Bondareva,
A. A. Baturin,
L. V. Lemasova,
N. N. Teteryatnikova,
L. I. Belitskaya
128-132 118
Abstract
Internal quality control of application of different reagent sets for in vitro diagnostics of the melioidosis agent and other closely related species of Burkholderia was organized and implemented to confirm the proper laboratory quality in the Reference centers for monitoring of glanders and melioidosis agents. The diagnostic capabilities of commercial and experimental kits for rapid and express-analysis (FIA, PCR, TIFM, RIHA) were evaluated during specific stages of the melioidosis agent indication. The test results of samples using PCR analysis confirmed the efficacy of the various reagent kits and permitted to conduct the indication, identification and differentiation of three species of closely related organisms for 5-6 hours.
132-134 149
Abstract
We compared several primer and probe sets developed for real-time PCR method enabling specific differentiation of glanders and melioidosis causative agents. The oligonucleotides constructed in this study were based on the sequence of the fliР gene and gp68-protein coding sequence for identification of B. mallei and B. pseudomallei, respectively.
134-137 126
Abstract
Detailed characteristic of experimental fluorescent immunoglobulins based on monoclonal antibodies against200 kDa antigen of Burkholderia pseudomallei was presented. This article contains information about its applicability for samples from experimental animal models.
137-139 116
Abstract
The conditions for performing a double immunodiffusion test using monoclonal antibodies (MAb) against antigen 200 kDa of Burkholderia pseudomallei were described. The variants of high specific MAbs were identified.
139-141 130
Abstract
Studies on the development of recent latex diagnosticum capable to identify Burkholderia pseudomallei were conducted. The concentration of blocking buffer was chosen. Optimal capacity of melioidosis immunoglobulins on polystyrene particles was 4 mg/ml. Analytical descriptions of the resulted diagnosticum were determined.
142-144 113
Abstract
Immunoblotting of total cellular proteins with sera to live cells and cellular proteins permitted to reveal antigens (molecular weight 20, 60, 88 kDa) in pathogenic burkholderias caused secretion of antibodies independently of an immunization variant and presumably located on cell surfaces.
144-146 98
Abstract
Colony morphology and culture virulence has changed at passage of 6 Burkholderia pseudomallei, 4 - B. mallei and 5 - B. thailandensis strains to animals and nutrient media. Antibiotic sensitivity of cultures passed to animals tended to increase but after a passage to nutrient media was noted differently directed change of resistance. Increase of culture virulence caused stabilization of colony morphology and in most cases reduced resistance to antibacterial preparations.
T. Yu. Zagoskina,
S. V. Balakhonov,
E. A. Chaporgina,
E. Yu. Markov,
O. B. Bodrykh,
T. A. Ivanova,
O. V. Gavrilova,
T. M. Dolgova,
T. S. Taikova,
Yu. O. Popova,
A. V. Korneva
146-149 300
Abstract
High-sensitive, specific test-system for dot-immunoassay is developed. This method permits to reveal botulotoxins of A, B, Е types within two hours in a clinical material from patients.
150-152 94
Abstract
Highly active and specific rabbit leptospirosis sera were prepared using live virulent and heat inactivated cultures as antigens. The stabilizer application increased expiration date of dry Leptospira sera till 5 years.
M. V. Podshivalova,
I. B. Zakharova,
Ya. A. Lopasteyskaya,
L. M. Verkina,
N. A. Selyanskaya,
D. V. Viktorov
152-154 134
Abstract
The results of the studies of antibiotic resistant Vibrio cholera strains isolated in Russia in 2009-2013 are presented. The presence of integrative element ICE of SXT/R391 family was detected trimethoprim/sulfamethoxazole-resistant strains. Using multilocus polymerase chain reaction the presence of SXTET variants carrying sullII, dfrA1sequence was revealed.
ISSN 2073-2899 (Print)
