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EVALUATION OF THE DIAGNOSTIC VALUE OF THE REAGENT KIT FOR DETECTION OF MELIOIDOSIS CAUSATIVE AGENT AT CONDUCTING OF INTERNAL QUALITY CONTROL OF THE LABORATORY RESEARCH IN THE REFERENCE CENTER FOR MONITORING OF GLANDERS AND MELIOIDOSIS AGENTS

Abstract

Internal quality control of application of different reagent sets for in vitro diagnostics of the melioidosis agent and other closely related species of Burkholderia was organized and implemented to confirm the proper laboratory quality in the Reference centers for monitoring of glanders and melioidosis agents. The diagnostic capabilities of commercial and experimental kits for rapid and express-analysis (FIA, PCR, TIFM, RIHA) were evaluated during specific stages of the melioidosis agent indication. The test results of samples using PCR analysis confirmed the efficacy of the various reagent kits and permitted to conduct the indication, identification and differentiation of three species of closely related organisms for 5-6 hours.

About the Authors

E. V. Prokhvatilova
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


V. A. Antonov
Volgograd State Medical University, Volgograd
Russian Federation


D. V. Viktorov
Volgograd State Medical University, Volgograd
Russian Federation


V. I. Ilyukhin
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


N. P. Khrapova
Volgograd State Medical University, Volgograd
Russian Federation


G. A. Tkachenko
Volgograd State Medical University, Volgograd
Russian Federation


I. B. Zakharova
Volgograd State Medical University, Volgograd
Russian Federation


N. G. Plehanova
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


I. V. Novitskaya
Volgograd State Medical University, Volgograd
Russian Federation


M. Ya. Kulakov
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


T. V. Zamarina
Volgograd State Medical University, Volgograd
Russian Federation


I. I. Korsakova
Volgograd State Medical University, Volgograd
Russian Federation


S. S. Savchenko
Volgograd State Medical University, Volgograd
Russian Federation


O. S. Bondareva
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


A. A. Baturin
Volgograd State Medical University, Volgograd
Russian Federation


L. V. Lemasova
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


N. N. Teteryatnikova
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


L. I. Belitskaya
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


References

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Review

For citations:


Prokhvatilova E.V., Antonov V.A., Viktorov D.V., Ilyukhin V.I., Khrapova N.P., Tkachenko G.A., Zakharova I.B., Plehanova N.G., Novitskaya I.V., Kulakov M.Ya., Zamarina T.V., Korsakova I.I., Savchenko S.S., Bondareva O.S., Baturin A.A., Lemasova L.V., Teteryatnikova N.N., Belitskaya L.I. EVALUATION OF THE DIAGNOSTIC VALUE OF THE REAGENT KIT FOR DETECTION OF MELIOIDOSIS CAUSATIVE AGENT AT CONDUCTING OF INTERNAL QUALITY CONTROL OF THE LABORATORY RESEARCH IN THE REFERENCE CENTER FOR MONITORING OF GLANDERS AND MELIOIDOSIS AGENTS. Far Eastern Journal of Infectious Pathology. 2014;(25):128-132. (In Russ.)

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ISSN 2073-2899 (Print)