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Far Eastern Journal of Infectious Pathology

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APPLICATION OF MALDI-TOF MASS SPECTOMETRY TO BRUCELLA SPP. IDENTIFICATION

Abstract

The project of refillable electronic database of mass spectrometric protein profiles of brucellosis strains in the environment of the program MALDI Biotyper v 3.0 (Bruker Daltonics, USA) has been developed. On the basis of analysis of representative mass spectra of 39 Brucella strains, 12 possible genus-specific fragments in the mass range 2000 - 20000 Da were identified.

About the Authors

D. V. Ulshina
Stavropol Plague Control Research Institute of Rospotrebnadzor, Stavropol
Russian Federation


D. A. Kovalev
Stavropol Plague Control Research Institute of Rospotrebnadzor, Stavropol
Russian Federation


S. I. Golovneva
Stavropol Plague Control Research Institute of Rospotrebnadzor, Stavropol
Russian Federation


E. V. Chebotareva
Stavropol Plague Control Research Institute of Rospotrebnadzor, Stavropol
Russian Federation


References

1. Ferreira L., Vega S., Sanchez-Juanes F. et al. Identifying bacteria using a matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer. Comparison with routine methods used in clinical microbiology laboratories // Enferm. Infecc. Microbiol. Clin. - 2010. - Vol. 28. - P. 492-497.

2. Lista F., Reubsaet FA, De Santis R et al. Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS. BMC Microbiology. - 2011. - Vol. 11. - P. 267.

3. Veen S., Claas E., Kuijper E. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories // J. Clin. Microbiol. - 2010. - Vol. 48. - P. 900-907.


Review

For citations:


Ulshina D.V., Kovalev D.A., Golovneva S.I., Chebotareva E.V. APPLICATION OF MALDI-TOF MASS SPECTOMETRY TO BRUCELLA SPP. IDENTIFICATION. Far Eastern Journal of Infectious Pathology. 2014;(25):111-113. (In Russ.)

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ISSN 2073-2899 (Print)