PECULIARITIES OF ENTEROVIRUS CIRCULATION DURING THE SEASON OF ENTEROVIRUS INFECTION INCIDENCE RAISE IN THE FAR EASTERN AND SIBERIAN FEDERAL DISTRICTS OF THE RUSSIAN FEDERATION IN THE YEAR 2019

Objective of the research – to reveal molecular-genetic and epidemiological peculiarities of different enterovirus types circulation in territories of the Far Eastern and Siberian federal districts (FEFD and SFD).

Materials and methods. Biological material obtained from people suspected to have enterovirus infection (EVI) and environmental samples were tested with method of polymerase chain reaction (PCR) in order to detect enterovirus RNA. In order to obtain enterovirus nucleotide samples and perform genotyping a Sanger sequencing technique with further phylogenetic analysis was utilized.

Results of the research. Data of the molecular-genetic research performed in the FEFD and SFD revealed that different enterovirus types caused the seasonal EVI incidence rate raise in 2019. Among revealed enteroviruses Coxsackie A-6 was prevalent in Khabarovsk, Primorsky and Krasnoyarsk regions, Jewish autonomous district and Irkutsk region, Altai Republic and Republic Sakha (Yakutia); Coxsackie A-16 virus was dominant in the Magadan and Amursk districts, Republic Khakasia; ECHO6 – in the Republic Tyva. In-depth phylogenetic analysis showed that the spread of one of the genetic lineages of ECHO-6 enterovirus in the territories of the Russian Federation including its importation in the Khabarovsk region and Republic Tyva took place not until 2018

Conclusion. The results of the conducted research confirmed importance of such component of epidemiological surveillance over EVI as molecular-genetic monitoring of enterovirus circulation carried out on an advanced level allowing to execute genotyping as well as reveal epidemiological connections between disease cases and suggest cross-border enterovirus importation more often from countries of the Asia-Pacific Region.

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