Molecular-genetic characteristics of chronic viral hepatitis B in patients of the Nanaysky district of the Khabarovsk region
Abstract
A molecular-genetic and serological analysis of 88 plasma samples collected from patients with diagnosis of chronic viral hepatitis B living on the territory of the Nanaysky district of the Khabarovsk region was performed. DNA of the hepatitis B virus (HBV) was detected in 49 (55.7±5.3%) samples. Based on the phylogenetic analysis of 48 nucleotide sequences it was shown that HBV genotype D was prevalent (81.2±5.6%, n=39) and was further divided in three subtypes: D3 (51.3±8.1%, n=20), D2 (46.1±8.1%, n=18) and D1 (2.6±2.6%, n=1). Genotype C was identified in 7 (14.6±5.1%) samples whereas genotype A in 2 (4.2±2.9%) specimens.
About the Authors
V. O. Kotova
FBIS Khabarovsk scientific research institute of epidemiology and microbiology of the Federal Service for surveillance on consumers rights protection and human wellbeing (Rospotrebnadsor)
Russian Federation
Russian Federation
Khabarovsk
L. A. Bakakhontseva
FBIS Khabarovsk scientific research institute of epidemiology and microbiology of the Federal Service for surveillance on consumers rights protection and human wellbeing (Rospotrebnadsor)
Russian Federation
Russian Federation
Khabarovsk
E. A. Bazykina
FBIS Khabarovsk scientific research institute of epidemiology and microbiology of the Federal Service for surveillance on consumers rights protection and human wellbeing (Rospotrebnadsor)
Russian Federation
Russian Federation
Khabarovsk
O. E. Trotsenko
FBIS Khabarovsk scientific research institute of epidemiology and microbiology of the Federal Service for surveillance on consumers rights protection and human wellbeing (Rospotrebnadsor)
Russian Federation
Russian Federation
Khabarovsk
V. N. Beldi
KGPFHCI “Troitsk central regional hospital”
Russian Federation
Russian Federation
Troitsk village, Nanaysky district, Khabarovsk region
References
1. Герасимова В.В., Максимова Н.Р., Левакова И.А., Мукомолов С.Л. Молекулярная эпидемиология вируса гепатита В в Якутии // Якутский медицинский журнал. –2014. – № 3(47). – С. 54-57.
2. Елпаева Е.А., Никитина О.Е., Писарева М.М., Шилова И.В. и др. Генетические варианты вируса гепатита В у пациентов с хроническим гепатитом В // Журнал Инфектология. - 2015. - т.7, №3. - C.44-50.
3. Ивашкин В.Т. Ющук Н.Д., Маевская М.В., Знойко О.О. и др. Клинические рекомендации Российской гастроэнтерологической ассоциации и Российского общества по изучению печени по диагностике и лечению взрослых больных гепатитом В // Российский журнал гастроэнтерологии, гепатологии и колопроктологии. – 2014. – Т.24, № 3. – С.58-88.
4. Кочнева Г.В., Мануйлов В.А., Нетесова И.Г., Чуб Е.В. и др. Генотипы и субгенотипы изолятов вируса гепатита В на территории Сибири // Проблемы Особо Опасных Инфекций. – 2011. - №3 (109). - С.31-35.
5. Кюрегян К.К., Михайлов М.И. Молекулярно-биологические основы контроля вирусных гепатитов. - М.: Издательство Икар, 2013. – 336 с.
6. Лукашев В.В. Молекулярная эволюция и филогенетический анализ. – М.: БИНОМ. Лаборатория знаний, 2009. – 256 с.
7. О состоянии санитарно-эпидемиологического благополучия населения в Российской Федерации в 2016 году: Государственный доклад. - М.: Федеральная служба по надзору в сфере защиты прав потребителей и благополучия человека, 2017. - 191 с.
8. Чуланов В.П. Эпидемиологическое и клиническое значение генетической гетерогенности вирусов гепатита А и В: автореф. дис. …д-ра. мед. наук: 14.02.02. – Москва, 2013. – 47 с.
9. Cui C., Shi J., Hui L. et al. The dominant hepatitis B virus genotype identified in Tibet is a C/D hybrid // J. Gen. Virol. – 2002. – Vol. 83. – P. 2773-2777.
10. Gao S. Clinical relevance of hepatitis B virus variants // World J. Hepatol.– 2015. – V. 7, № 8 – P.1086-1096.
11. Gerlich W.H. Medical virology of hepatitis B: how it began and where we are now // Virol. J. – 2013. – V. 10, №1. – P.2-25.
12. Hepatitis B Foundation. Hepatitis B Foundation [Internet]. Doylestown, PA. Available from: http://www.hepb.org/.
13. Emekdas G., Tezcan S., Aslan G. et al. Determination of hepatitis B virus genotypes in chronic hepatitis B patients in Mersin province, Turkey // Mikrobiyol. Bul. – 2012. – Vol. 46 (3). – P. 432-445.
14. Lin C.L., Kao J.H. The clinical implications of hepatitis B virus genotype: Recent advances // J. Gastroenterol. Hepatol. – 2011. – Suppl. 1. – P. 123-130.
15. Lin C.L., Kao J.H. Hepatitis B Virus Genotypes and Variants // Cold Spring Harb. Perspect. Med. – 2015. – V. 5, № 5 – P.a021436–a021436. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4448583/).
16. Lozano R., Naghavi M., Foreman K., Lim S. et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010 //Lancet. – 2012. - V.380. - Р.2095–2128.
17. Magnius L.O., Norder H. Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene //Intervirology – 1995. – V. 38, № 1-2. – P.24–34.
18. Norder H., Courouce A.M., Magnius L.O. Molecular basis of hepatitis B virus serotype variations within the four major subtypes // J. Gen. Virol. – 1992. – V.73.- P.3141–3145.
19. Norder H, Courouce A.M, Coursaget P, et al. Genetic diversity of hepatitis B virus strains derived worldwide: genotypes, subgenotypes, and HBsAg subtypes // Intervirol. – 2004. –V.47. - Р.289–309.
20. Okamoto H., Tsuda F., Sakugawa H., Sastrosoewignjo RI. et al. Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes // J. Gen. Virol. – 1988. – Vol. 69 (Pt 10). – P. 2575-2583.
21. Tatematsu K. A genetic variant of hepatitis B virus divergent from known Human and ape genotypes isolated from a Japanese patient and provisionally assigned to new genotype J // J. Virol. – 2009. – V.83(20). – P.10538-10547.
22. Wang Z., Liu Z., Zeng G. et al. A new intertype recombinant between genotypes C and D of hepatitis B virus identified in China // J. Gen. Virol. –2005. – Vol. 86. – P. 985-990.
23. World Health Organization. Hepatitis B. Fact sheet. (http://www.who.int/en/news-room/factsheets/detail/hepatitis-b)
Review
For citations:
Kotova V.O., Bakakhontseva L.A., Bazykina E.A., Trotsenko O.E., Beldi V.N. Molecular-genetic characteristics of chronic viral hepatitis B in patients of the Nanaysky district of the Khabarovsk region. Far Eastern Journal of Infectious Pathology. 2018;(35):15-21. (In Russ.)
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