Molecular-epidemiologic analysis of enteroviruses circulating on the territory of the Far Eastern and Siberian Federal Districts of the Russian Federation including those that caused outbreaks of the disease
Abstract
Enteroviruses are spread ubiquitously and under certain circumstances can cause sporadic cases of the disease or breakouts of enterovirus infection (EVI) that have different clinical manifestations. Molecular-biological approach allowed to identify 32 enterovirus types that were circulating in the constituent entities of the Far Eastern and Siberian Federal Districts during year 2017. The most prevalent viruses that caused highest incidence were Coxsackie A-10, Coxsackie A-6 and Coxsackie A-9. The conducted evolutionary analysis allowed to identify 2 lineages of Coxsackie A-10 and 3 groups of Coxsackie A-6 that were presented as genotype D. Etiological origin of EVI breakouts in children organized communities in Irkutsk region and Krasnoyarsk region in 2017 were Coxsackie A-6, Coxsackie A-9 and ECHO-18. The obtained results of the phylogenetic research allowed us to suspect that these viruses were introduced on the territories of the Russian Federal Districts from the Southeast Asian nations. Absent levels of immunity against current viral serotypes among population of the Irkutsk and Krasnoyarsk regions lead to breakouts. The obtained data indicate on a necessity of ongoing molecular-epidemiological surveillance over enteroviruses in supervised territories.
About the Authors
E. Yu. SapegaRussian Federation
Khabarovsk
L. V. Butakova
Russian Federation
Khabarovsk
O. E. Trotsenko
Russian Federation
Khabarovsk
D. V. Goryaev
Russian Federation
Krasnoyarsk
G. M. Dmitrieva
Russian Federation
Krasnoyarsk
T. N. Detkovskaya
Russian Federation
Vladivostok
N. I. Zhdanova
Russian Federation
Petropavlovsk-Kamchatsky
T. A. Zaitseva
Russian Federation
Khabarovsk
M. E. Ignatyeva
Russian Federation
Yakutsk
O. P. Kurganova
Russian Federation
Blagoveshchensk-on-Amur
P. V. Kopilov
Russian Federation
Birobidzhan
S. E. Lapa
Russian Federation
Chita
A. N. Perezhogin
Russian Federation
Irkutsk
A. A. Rubtsova
Russian Federation
Magadan
O. A. Funtusova
Russian Federation
Yuzhno-Sakhalinsk
S. S. Khankhareev
Russian Federation
Ulan-Ude
L. V. Shuchinov
Russian Federation
Gorno-Altaysk
References
1. Бутакова Л.В., Троценко О.Е., Сапега Е.Ю. Энтеровирусная инфекция: обзор ситуации в мире на современном этапе в условиях активизации миграционных процессов // Здоровье населения и среда обитания. – 2018. – № 4(301). – С. 55–60.
2. Сапега Е.Ю., Бутакова Л.В., Котова В.О. и др. Молекулярно-биологические особенности циркуляции энтеровирусов в Дальневосточном федеральном округе Российской Федерации В 2014- 2015 годах // Дальневосточный журнал инфекционной патологии. – 2016. – №30. – С. 38–44.
3. Сапега Е.Ю., Бутакова Л.В., Троценко О.Е. Особенности эпидемиологической ситуации по энтеровирусной инфекции в Дальневосточном и Сибирском федеральных округах в 2017 году // Информационный бюллетень. – 2018. – Том 5. – С. 18–23. Электронный ресурс: http://www.nniiem.ru/file/razrabotki/2018/byulleten-evi-2017-n5-may-18-1.pdf
4. Drummond A.J., Rambaut A. BEAST: Bayesian evolutionary analysis by sampling trees // BMC Evol Biol – 2007. – Vol. 7. – P. 214.
5. Lukashev A.N., Drexler J.F., Kotova V.O. et al. Novel serotypes 105 and 116 are members of distinct subgroups of human enterovirus C. The Journal of General Virology – 2012. – Vol. 93 (11). – P. 2357- 62.
6. Nix W.A, Oberste M.S, Pallansch M.A. Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens. // J. Clin. Microbiol. – 2006. – Vol. 44. – № 8. – P. 2698-704.
7. Shapiro B, Rambaut A, Drummond A.J. Choosing appropriate substitution models for the phylogenetic analysis of protein-coding sequences. // Mol Biol Evol. – 2006. – Vol. 23(1). – P. 7–9.
Review
For citations:
Sapega E.Yu., Butakova L.V., Trotsenko O.E., Goryaev D.V., Dmitrieva G.M., Detkovskaya T.N., Zhdanova N.I., Zaitseva T.A., Ignatyeva M.E., Kurganova O.P., Kopilov P.V., Lapa S.E., Perezhogin A.N., Rubtsova A.A., Funtusova O.A., Khankhareev S.S., Shuchinov L.V. Molecular-epidemiologic analysis of enteroviruses circulating on the territory of the Far Eastern and Siberian Federal Districts of the Russian Federation including those that caused outbreaks of the disease. Far Eastern Journal of Infectious Pathology. 2018;(35):6-14. (In Russ.)