Modern methods of detection and identification of enteroviruses

Abstract

Present review paper is dedicated to theoretical basics, methodological principles, significance and applicability of modern molecular-genetic methods of detection and identification of enteroviruses in practical virology. Modern approaches allow to identify molecular structure of viral particles, ways of cell penetration and peculiarities of viral reproduction as well as analysis of primary structure of viral protein and viral nucleic acids. Among these approaches are polymerase chain reaction (PCR) and analysis of genome nucleic acid sequences that code proteins (sequencing). These methods allow to identify in the sampling material ultimate particles of viral nucleic acids, help to determine the cause of intracellular processes what is particularly significant in differential diagnosis.