Results of investigation of HIV-1tropism in HIV-infected patients on the territory of Far Eastern Federal District

The results of investigation of HIV tropism to coreceptor, by genotyping based on analysis of nucleotide sequence of env V3-loop, in HIV-infected patients of the Far Eastern Federal District, who did not receive CCR5 antagonist, are presented in this study. A comparative analysis of R5 and X4 tropic HIV viruses spread among treated and untreated patient with antiretroviral therapy was performed. Possible relations of R5 and X-4tropic variants of HIV infection, duration of infection, CD4 rate and total load were found.

ВИЧ-инфекция, генотипическая методика, тропизм, CCR-5, CXCR4, антиретровирусая терапия (АРВТ), антагонисты CCR5 корецепторов, HIV infection, genotyping method, tropism, CCR-5, CXCR4, antiretroviral therapy, antagonists of CCR5 coreceptors

1. Бобкова М.Р. Лекарственная устойчивость ВИЧ. - М.: Человек, 2014. - 164 с.

2. Зайцева Н.Н., Ефимов Е.И., Носов Н.Н., Парфенова О.В., Пекшева О.Ю. Современные молекулярно-генетические методы исследования в эпидемиологическом надзоре за ВИЧ-инфекцией (Аналитический обзор) // Электронный «Журнал МедиАль» (www.mediall-journal.ru), 2014. - № 2 (12). - С.128.

3. Кравченко А.В. Применение препарата маравирок - первого антагониста рецепторов CCR5 - в схемах терапии ВИЧ-инфекции // Тер. архив. - 2013. - №85(11). - С.125.

4. Лопатухин А.Э., Киреев Д.Е., Поляков А.Н., Букин Е.К., Kaiser R., Luebke N., Куевда Д.А., Шипулин Г.А. Первый опыт применения стандартизированной генотипической методики определения тропизма ВИЧ // Клин. лаб. диагн. - 2013. - №6. -С.46-48.

5. Хоффман К., Рокштро Ю.К. Лечение ВИЧ-инфекции. - М.: Р. Валент, 2012. - С. 330-331.

6. Bennetts В.H., Teutsch S.M, Buhler M.M, Heard R.N, Stewart G.J. The CCR5 deletion mutation fails to protect againstmultiple sclerosis // Human Immunology.- 1997. - V. 58. - P. 52-59.

7. Berger E.A., Murphy P.M., FarberJ.M. Chemokine receptors as HIV-1 coreceptors: Roles in viral entry, tropism, and disease // Annu. Rev. Immunol. - 1999. - V.17. - P. 657-700.

8. Dean М., Carrington M., Winkler C., Huttley G.A., Smith M.W., Allikmets R., Goedert J.J., Buchbinder S.P., Vittinghoff E., Gomperts E., Donfield S., Vlahov D., Kaslow R., Saah A., Rinaldo C., Detels R., O'Brien S.J. Genetic restriction of HIV-1 infection and progression to AIDS by a deletion allele of the CСR5 structural gene // Science. - 1996. - V. 273. - P. 1856-1862.

9. Dragic T. An overview of the determinants of CCR5 and CXCR4 co-receptor function // J. of Gen. Virol. - 2001. - V. 82. - P. 1807-1814.

10. Michael M. Thomson, Elena Vázquez de Parga, Anna Vinogradova, María Sierra, Aleksey Yakovlev, Aza Rakhmanova, Elena Delgado, Gema Casado, Mercedes Muñoz, Rocío Carmona, Yolanda Vega, Lucía Pérez-Álvarez, Gerardo Contreras, Leandro Medrano, Saladin Osmanov, Rafael Nájera. New Insights into the Origin of the HIV Type 1 Subtype A Epidemic in Former Soviet Union's Countries Derived from Sequence Analyses of Preepidemically Transmitted Viruses // AIDS Research and Human Retroviruses. - December 2007. - V. 23(12). - Р. 1599. -1604.

11. Jenkins C., Robalino D.A. HIV/AIDS in the Middle East and North Africa: Тhe Costs of Inaction. - Washington: World Bank, 2003.

12. Jiang C., Parrish N.F., Wilen C.B., Li H., Chen Y, Pavlicek J.W., Berg A., Lu X., Song H., Tilton J.C., Pfaff J.M., Henning E.A., Decker J.M., Moody M.A., Drinker M.S., Schutte R., Freel S., Tomaras G.D., Nedellec R., Mosier D.E., Haynes B.F., Shaw G.M., Hahn B.H., Doms R.W., Gao F. Infection by a Human Immunodeficiency Virus with Atypical Coreceptor Tropism // J Virol. - 2011. - V. 10. - Р.10669-10681.

13. Wang Z.X., Berson J.F., Zhang T.Y., Cen Y.H., Sun Y., Sharron M., Lu Z.H., Peiper S.C. CXCR4 sequences involved in coreceptor determination of human immunodeficiency virus type-1 tropism. Unmasking of activity with M-tropic Env glycoproteins // J. Biol. Chem.- 1998. - V. 273. - P. 15007-15015.