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Far Eastern Journal of Infectious Pathology

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DEVELOPMENT OF DIAGNOSTICUM FOR THE DETECTION OF MELIOIDOSIS AGENTS IN LATEX AGGLUTINATION REACTION

Abstract

Studies on the development of recent latex diagnosticum capable to identify Burkholderia pseudomallei were conducted. The concentration of blocking buffer was chosen. Optimal capacity of melioidosis immunoglobulins on polystyrene particles was 4 mg/ml. Analytical descriptions of the resulted diagnosticum were determined.

About the Authors

A. M. Kudelina
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


I. V. Novitskaya
Volgograd State Medical University, Volgograd
Russian Federation


E. V. Prokhvatilova
Volgograd Plague Control Research Institute, Volgograd
Russian Federation


References

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2. Шубин Г.Г., Ларионова Л.В., Карбышев Г.Л. и др. Конструирование и испытания листериозного антигенного полимерного диагностикума // Биотехнология. - 2009. - № 3. - С. 91-95.

3. Duval B.D., Elrod M.G., Gee J.E. et al. Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia pseudomallei and Burkholderia mallei // The American journal of tropical medicine and hegiene - 2014. - 14 (0025). - С. 48

4. Garcia V.S., Gonzalez V.D., Marcipar I.S. et al. Optimisation and standardisation of an immunoagglutination assay for the diagnosis of Trypanosomacruzi infection based on latex-(recombinant antigen) complexes // Tropical medicine and internatiol Health. - 2014. - 19(1) - P. 37-46.


Review

For citations:


Kudelina A.M., Novitskaya I.V., Prokhvatilova E.V. DEVELOPMENT OF DIAGNOSTICUM FOR THE DETECTION OF MELIOIDOSIS AGENTS IN LATEX AGGLUTINATION REACTION. Far Eastern Journal of Infectious Pathology. 2014;(25):139-141. (In Russ.)

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ISSN 2073-2899 (Print)