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Far Eastern Journal of Infectious Pathology

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Application of monoclonal antibodies for detection OF Burkholderia pseudomallei antigen 200 kDa by double immunodiffusion test

Abstract

The conditions for performing a double immunodiffusion test using monoclonal antibodies (MAb) against antigen 200 kDa of Burkholderia pseudomallei were described. The variants of high specific MAbs were identified.

About the Authors

T. V. Zamarina
Volgograd Plague Control Research Institute
Russian Federation


N. P. Khrapova
Volgograd; Volgograd State Medical University, Volgograd
Russian Federation


I. I. Korsakova
Volgograd; Volgograd State Medical University, Volgograd
Russian Federation


References

1. Корсакова И.И., Храпова Н.П., Напалкова Г.М. и др. Сравнительный анализ иммунохимических методов исследования антигенов патогенных буркхольдерий // Проблемы особо опасных инф.- 2012.- Вып. 113.- С. 82-85.

2. Лабораторная диагностика опасных инфекционных болезней: Практическое руководство / Под редакцией академика РАМН Г.Г. Онищенко, академика РАМН В.В. Кутырева. Изд. 2-е, переработанное и дополненное. М.: ЗАО «Шико», 2013. - 560 с.

3. Anuntagool N., Panichakul T., Aramsri P., Sirsinha S. Shedding of lipopolysaccharide and 200-kDa surface antigen during the in vitro growth of virulent Ara- and avirulent Ara+ Burkholderia pseudomallei // Acta Tropica. - 2000. - V. 74. - P. 221-228.

4. Use of monoclonal antibody to increase sensitivity and specificity in quantitative immunodiffusion assay/ Glodel M., Montgomery R. R., Smith C.G. et al. // J. Immunol. Meth. - 1982. - Vol.48, N1. - P. 13-22.


Review

For citations:


Zamarina T.V., Khrapova N.P., Korsakova I.I. Application of monoclonal antibodies for detection OF Burkholderia pseudomallei antigen 200 kDa by double immunodiffusion test. Far Eastern Journal of Infectious Pathology. 2014;(25):137-139. (In Russ.)

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ISSN 2073-2899 (Print)