EVALUATION OF PCR PRIMERS AND PROBES FOR BURKHOLDERIA mallei AND BURKHOLDERIA pseudomallei DIFFERENTIATION
Abstract
We compared several primer and probe sets developed for real-time PCR method enabling specific differentiation of glanders and melioidosis causative agents. The oligonucleotides constructed in this study were based on the sequence of the fliР gene and gp68-protein coding sequence for identification of B. mallei and B. pseudomallei, respectively.
About the Authors
L. V. Lemasova
Volgograd Plague Control Research Institute of Rospotrebnadzor, Volgograd
Russian Federation
Russian Federation
S. S. Savchenko
Volgograd Plague Control Research Institute of Rospotrebnadzor, Volgograd
Russian Federation
Russian Federation
G. A. Tkachenko
Volgograd Plague Control Research Institute of Rospotrebnadzor, Volgograd
Russian Federation
Russian Federation
V. A. Antonov
Volgograd Plague Control Research Institute of Rospotrebnadzor, Volgograd
Russian Federation
Russian Federation
References
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Review
For citations:
Lemasova L.V., Savchenko S.S., Tkachenko G.A., Antonov V.A. EVALUATION OF PCR PRIMERS AND PROBES FOR BURKHOLDERIA mallei AND BURKHOLDERIA pseudomallei DIFFERENTIATION . Far Eastern Journal of Infectious Pathology. 2014;(25):132-134. (In Russ.)
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