DEVELPOPMENT OF POLYMERASE CHAIN REACTION KIT FOR DETECTION OF CORONAVIRUS CAUSING MIDDLE EASTERN RESPIRATORY SYNDROME

Abstract

A kit comprising the key components solely of Russian manufacturers and vendors for PCR and detection of Middle Eastern respiratory syndrome coronavirus 1b gene was developed on the basis of the primers and a probe suggested by German Center for the Study of Infections (Deutsches Zentrum für Infektionsforschung, DZIF, Germany). The detection of a virus-specific amplicon was performed by measuring the fluorescence as the endpoint and the ultraviolet light after the gel electrophoresis in the presence of ethidium bromide. The reaction was conducted in an accelerated program in 15 (10) μl volume. This approach does not require expensive equipment and Taq DNA polymerase such as Platinum-Taq DNA polymerase.